Cardiovascular Surgery Instrument Kit

Cardiovascular surgery kits focus on the heart and blood vessels. Cardiac surgery kit is mainly used to treat the complexities of ischemic heart diseases, congenital heart diseases, valvular heart diseases, endocarditis, rheumatic heart diseases, and atherosclerosis.

$1,700.00

Description

Mouse Kit
Operating Scissors (Round Type)S/S Str/11.5cm
IRIS-Fine Scissors (Round Type)S/S Str/9.5cm
Spring Scissors (Triangular)S/S Str/5*0.1mm/8.5cm
IRIS Dissecting Forceps-LargeCvd, 0.8mm Tips, 10cm
Dressing ForcepsStr, 1.8mm Tips, 9cm
HARTMAN Mosquito ForcepsStr, 1.0mm Tips, 10cm
HARTMAN Mosquito ForcepsCvd, 1.0mm Tips, 10cm
PGA Sutures w/Needleo1/2/4×10/90cm/5-0 (50/Box
Sutures w/Needle △3/8/2.5×7/30cm/6-0 (50/Box)
STEVENS Hooks, 1 Angled Tooth(5mm long), 12.5cm
3×3 Teeth Retractors-Blunt4.5cm
OLSEN-HEGAR Needle Holders with ScissorsStr, 12cm
SS Micro ClampsStr/L*W 4*0.75mm/13mm
Clip Applicator for R31005- and R31006-Clamps14cm
Spinal Cord HookTip Dia. 3mm/12cm
Instrument Storage Portfolio32*22cm
Rat Kit
Operating Scissors (Round Type)S/S Str/12.5cm
IRIS-Fine Scissors (Round Type)S/S Str/10.5cm
Spring Scissors (Triangular)S/S Str/5*0.1mm/8.5cm
IRIS Dissecting Forceps-LargeCvd, 0.8mm Tips, 10cm
IRIS Dissecting ForcepsStr, 0.8mm Tips, 10cm
HALSTED Mosquito ForcepsStr, 1.0mm Tips, 12.5cm
HALSTED Mosquito ForcepsCvd, 1.0mm Tips, 12.5cm
PGA Sutures w/Needleo1/2/4×10/90cm/5-0 (50/Box)
Sutures w/Needle△3/8/2.5×7/30cm/6-0 (50/Box)
STEVENS Hooks, 1 Angled Tooth (5mm long)12.5cm
3×3 Teeth Retractors-Blunt4.5cm
OLSEN-HEGAR Needle Holders with ScissorsStr, 12cm
SS Micro ClampsStr/L*W 4*0.75mm/13mm
Spinal Cord HookTip Dia. 3mm/12cm
Clip Applicator for R31005- and R31006-Clamps14cm
Instrument Storage Portfolio32*22cm
 
Introduction

Cardiovascular surgery is mainly performed to treat the complexities of ischemic heart diseases, congenital heart diseases, valvular heart diseases, endocarditis, rheumatic heart diseases, and atherosclerosis. It also includes heart transplantation.

The preferred rodent species for conducting cardiovascular research is shifting from rat to mouse. The main advantage of using the rat is its bigger size. However, the development of more sophisticated micro-dissecting microscopes and imaging devices, high-caliber microsurgical instruments, small catheters for hemodynamic measurements, etc., has made microsurgery in the mouse more feasible than rats. Mouse models mimicking human diseases are essential tools in biomedical research, which aim to understand the underlying mechanisms of many disease states. Mouse models have gained popularity because of their small size, rapid gestation age (21 days), relatively low husbandry costs, and convenience in housing and handling. Moreover, the extensively characterized mouse genome and gene-targeting (i.e., knockout) and transgenic overexpression experiments are widely performed using mice rather rats (Tarnavski, 2009).

 

Pre-operative Set-up and Anesthesia Induction

Thoroughly clean and sterilize the instruments used for the surgery.  Autoclave and disinfect the equipment. Clear the operating area of any disturbances and ensure asepsis. Before the surgery, record the subject identification details such as strain and gender, most importantly note the weight of the subject. Also, examine the subject physically to assess its health status and activeness. Ensure that the subject has been acclimated to the facility appropriately. The acclimation process can last from a few days to a couple of weeks.

Anesthesia

  • Anesthetize the surgical subjects with pentobarbital 70 mg/kg in a solution of 15 mg/ml (Use a face mask or an anesthetic chamber to anesthetize the animals). Do not administer analgesics pre-operatively.
  • Shave the chest with a hair clipper or a razor.
  • Place the animal on the surgical stage in surgical position for the subsequent intubation, wire 3-0 silk behind the front incisors, pull taut and fix with tape.
  • Intubate the animal with a 20-ga catheter, attached to the extender.
  • Connect the intubation tube to the ventilator and start ventilating the animal.
  • Perform the surgical procedure at room temperature (except for the ischemia-reperfusion).
  • Prepare the surgical field.

 

Cardiovascular Surgery Protocols

Mouse Cardiac Surgery Protocol (Tarnavski et al., 2004)

  • Open the chest with a lateral incision at the 4th intercostal space on the left side of the sternum and retract the chest.
  • Remove the pericardial sac and pass an 8-0 silk suture in the myocardium at the anterior surface of the heart for one second and then remove it.
  • Close the chest wall by approximating the third and fourth ribs with one or two interrupted sutures, return the muscles to their original position, and the skin closed with retract4-0 prolene suture.
  • Gently disconnect the animal from the ventilator.

 

Aortic Banding (Pressure-overload Model) Method (Tarnavski, 2009)

  • Locally anesthetize the animal by injecting 0.1 ml of 0.2% lidocaine subcutaneously at the surgical site.
  • Transversely incise the skin 5-mm with scissors 1–2 mm higher than the level of the ‘‘armpit’’ (with paw extended at 90o) 2 mm away from the left sternal border.
  • Separate the two layers of thoracic muscles.
  • Separate the intercostal muscles in the 2d intercostal space.
  • Interpolate the chest retractor to facilitate the view.
  • Pull the thymus and surrounding fat behind the left arm of the retractor. Gently pull the pericardial sac and attach it to both arms of the retractor.

The steps mentioned above remain the same for both ascending aortic constriction and transverse aortic constriction.

Ascending Aortic Constriction (AAC)

  1. Bluntly dissect the ascending portion of the aorta on its lateral side from the pulmonary trunk with Foerster curved forceps.
  2. Place Foerster curved forceps from the medial side under the ascending aorta, hold the 7-0 silk suture on the opposite side and pass it underneath the aorta.
  3. Tie a loose double knot to create a loop 7–10mm in diameter.
  4. Position a needle of proper size into the circuit.
  5. Tie the loop around the aorta and needle, and bind with the second knot; immediately remove the needle to provide a lumen with a stenotic aorta. Make another knot to secure the tie.
  6. Remove the chest retractor and re-inflate the lungs.
  7. Close the chest wound layer-by-layer.

Transverse Aortic Constriction (TAC)

  1. Bluntly depart the thymus, and pericardial sac with slightly curved forceps, then separate the aortic arch from the surrounding tissues and vessels.
  2. With the help of a blunted needle, create a way underneath the aortic arch.
  3. With a ‘‘wire and snare’’ device, deliver a 7-0 silk suture underneath the aortic arch between the innominate and left carotid arteries.
  4. Tie a loose double knot to create a loop 7–10mm in diameter.
  5. Position a needle of proper size into the circuit.
  6. Tie the circle around the aorta and needle, and secure with the second knot; remove the needle immediately to provide a lumen with a stenotic aorta. Make another knot to bind the tie.
  7. Remove the chest retractor and re-inflate the lungs.

 

Pulmonary Artery Banding (Right Ventricular Pressure-overload Model) Method

The principle of pulmonary artery banding is similar to that of aortic banding. However, while performing this surgery, be careful of specific challenges.

  1. Approach the great vessels via the second intercostal space.
  2. To supplement the animal with local anesthesia inject 0.1 ml of 0.2% lidocaine subcutaneously at the surgical site.
  3. Make a transverse 5-mm incision of the skin with scissors 1–2 mm higher than the level of the ‘‘armpit’’ (with paw extended at 90o) 2 mm away from the left sternal border.
  4. Separate the two layers of thoracic muscle.
  5. Isolate the intercostal muscles in the 2d intercostal space.
  6. Interpolate the chest retractor to visualize the organs.
  7. Pull the thymus and surrounding fat behind the left arm of the retractor. Gently draw the pericardial sac and attach it to both the arms of the retractor.
  8. After moving the pericardium, observe the pulmonary trunk (partially covered by the left atrium).
  9. With the help of Foerster curved forceps, bluntly dissect the pulmonary trunk from the aorta (on the left) and left atrium (on the right).
  10. With a blunted needle, create a passage under the pulmonary trunk.
  11. With the help of a ‘‘wire and snare’’ device, move the 7-0 silk suture underneath the pulmonary trunk.
  12. Tie a loose double knot to create a loop 7–10mm in diameter.
  13. Position an appropriately sized needle into the loop.
  14. Tie the loop around the aorta and needle and secure with the second knot; immediately remove the needle to provide a lumen with a stenotic pulmonary artery. Make another knot to secure the tie.
  15. As the heart rate significantly slows down, stimulate the mouse.
  16. Remove the chest retractor and re-inflate the lungs.
  17. Close the chest wound layer-by-layer.

 

Myocardial Infarction Model (Permanent Ligation of the Artery) Protocol

  1. For local anesthesia, inject 0.1 ml of 0.2% lidocaine subcutaneously at the surgical site.
  2. Make an oblique 8-mm incision of the skin (parallel to ribs) 2 mm far from the left sternal border toward the left armpit (1–2 mm below it).
  3. Separate the two layers of the thoracic muscle.
  4. Separate the intercostal muscles in the fourth intercostal space.
  5. Insert the chest retractor to facilitate the view.
  6. Gently pull apart the pericardial sac and attach it to both arms of the retractor.
  7. Locate the left anterior descending (LAD) coronary artery.
  8. Pass the tapered needle with 7-0 silk suture underneath the LAD.
  9. Tie the suture with one double and then with one single knot.
  10. Remove the chest retractor and close the wound layer-by-layer.

 

Ischemia-Reperfusion Model Protocol

  1. Hold the artery and place the suture (same as step 1-8 above).
  2. To create ischemia, tie the temporary suture around the LAD. Cover the incision with wet gauze to prevent drying.
  3. Release the tie (do not remove the suture) at the end of ischemia and observe the reperfusion.
  4. If the procedure is survival, leave the suture underneath the LAD and close the chest wound layer-by-layer.
  5. Retie the suture to demarcate the area at risk by infusion of blue dye.

 

Coronary Artery Ligation and Intra-myocardial Injection in a Murine Model of Infarction Protocol (Virag & Lust, 2011)

  • Intubate the animals intratracheally by transesophageal illumination using a fiber-optic light.
  • Connect the tubing to the ventilator.
  • Expose the pectoral muscles by making an incision on the chest to view the ribs.
  • For ischemia/reperfusion studies, place a 1 cm piece of PE tubing over the heart to tie the ligature to so that occlusion/reperfusion can be customized.
  • For intramyocardial injections, use a syringe with a sterile 30gauge beveled needle.
  • Complete the myocardial manipulations, close the rib cage, the pectoral muscles, and the skin sequentially.
  • Apply 0.25% Bupivacaine in sterile saline to muscle layer before the closure of the skin.
  • Give a subcutaneous injection of saline and place the mice in a warming chamber until they are sternally recumbent.
  • Return the animals to the vivarium and housed under standard conditions until the time of tissue collection.
  • Anesthetize the mice, arrest the heart in diastole with KCl or BDM, rinse with saline, and immerse in fixative.
  • Subsequently, perform routine procedures for processing, embedding, sectioning, and histological staining.

 

Postoperative Management

  • Administer the first dose of the analgesic (0.1 mg/kg) intraperitoneally at the completion of the surgery.
  • Move the animal to another ventilator in the recovery area with 100% oxygen loosely connected to its inflow.
  • Provide heat by either a 60-W lamp or a heating pad at a low setting.
  • Once the mouse attempts to breathe spontaneously (generally after 45–60 min), disconnect the intubation tube from the ventilator.
  • Keep the intubation tube in the trachea for another 10–15 min until the mouse resumes the regular breathing pattern.
  • Provide supplementary oxygen (the mouse is placed next to the source of oxygen).
  • Extubate the mouse and return it to a clean, warm cage.

 

Applications

Genomic Study of Coronary Diseases in Murine Models

Mouse models mimicking human cardiac diseases are essential paradigms to explain the underlying mechanisms of many disease states. Several surgical models have been developed that mimic human myocardial infarction (MI) and pressure-overload-induced cardiac hypertrophy. Microarray technologies, used in the research, measure the expression of thousands of genes simultaneously, allowing for the identification of genes and pathways that may potentially be involved in the disease process. In the study, a description of three major surgical procedures has been discussed: 1) aortic constriction, 2) pulmonary artery banding, 3) MI (including ischemia-reperfusion).  The cardiac surgery techniques mentioned have been and will continue to be, essential for elucidating the molecular mechanisms of cardiac hypertrophy and genome profiling.

Evaluation of Galectin-1: a Biomarker of Surgical Stress in Murine Model of Cardiac Surgery (Hashmi & Al-Salam, 2015)

Galectin-1 (GAL-1) is a member of the β-galactoside-binding lectins family. GAL-1 regulates cell-cell and cell-matrix interactions, the immune response, apoptosis, cell cycle, RNA splicing, and neoplastic transformation. Satwat and Suhail investigated the effect of heart manipulation secondary to cardiac surgery on the level of GAL-1 in murine heart and plasma. They used male C57B6/J mice for the adopted model of cardiac surgery. Then the heart samples were processed for immunohistochemical and immunofluorescent labeling; Enzyme-linked immunosorbent assay and quantitative RT-PCR to identify GAL-1 levels in the heart and plasma during the first 24 hours following cardiac surgery. Results suggested that the GAL-1 is a valuable biomarker of surgical stress.

Evaluating Coronary Artery Ligation and Intramyocardial Injection in a Murine Model of Infarction

Mouse models are widely used to study acute injury and chronic remodeling of the myocardium in vivo. With the advancement of genetic modifications to the whole organism or the myocardium and an array of biological and synthetic materials, there is an excellent potential for any combination of these to mitigate the extent of the acute ischemic injury and impede the onset of heart failure according to myocardial remodeling.

The study presented the methods and materials used to perform the microsurgery reliably and the modifications for temporary (with reperfusion) or permanent coronary artery occlusion studies as well as intramyocardial injections. Development of miniature technology for imaging in vivo, analyzing large-scale genomics and proteomics, drug screening, efficacy of cell-based and/or protein therapies as well as biomaterials, combined with the increasingly wide range of genetic manipulations afforded by ubiquitous or tissue-specific transgenic or mutant/knockout mice, the murine model of myocardial infarction, undoubtedly, continue to be an invaluable tool in evaluating acute cardiac injury and long-term remodeling. Therefore, there is an absolute value in being able to perform these experiments reliably and reproducibly.

 

Precautions

Murine cardiac surgical procedures are relatively short, so it is not necessary to withhold food and water from mice before the surgery. Before starting the surgical procedure, ensure the adequate depth of the anesthesia using a toe pinch test or other anesthesia assessment tests. It is important not to disturb or agitate the animal while the anesthetic is taking effect to ensure smooth induction and facilitate subsequent procedures. Do not administer pre-surgical analgesia (buprenorphine) since narcotic analgesics are known to depress the respiratory center and may interfere with the survival after the open-chest surgery. Since the described surgical procedures are relatively short (15–20 min), it is not critical to strictly regulate the core temperature of the mouse. Do not pull apart the pericardial sac with too much force as it may rupture the wall of the left superior vena cava and cause bleeding that can be fatal. The significant challenges that arise during the cardiac surgery are because of the extremely thin and delicate walls of the pulmonary trunk and the incapacity of the right ventricle to undergo stress while the pulmonary artery is being manipulated. Carry out the whole surgical procedure under aseptic conditions.

 

Strengths and Limitations of Rodent Models for Cardiac Surgery

The most widely used rodent species for conducting cardiovascular research is the mouse. The significant advantage of using the rat is its bigger size. However, the development of micro-dissecting microscopes and other imaging devices, high-caliber microsurgical instruments, small catheters for hemodynamic measurements, etc., has made microsurgery in the mouse as feasible as in the rat. Mouse models have gained popularity because of their small size, rapid gestation age (21 days), relatively low maintenance costs, convenience in housing and handling, and fewer compound requirements for pharmacological studies. Use of a simple dissecting scope or magnifying glass and well-lit conditions enable the vasculature to be seen readily. To exterminate the risk of postoperative mortality, it is essential to avoid severing large vessels since the total blood volume of a 25g mouse is less than 2ml. If excessive bleeding occurs, gentle application of pressure or pinpoint cauterization can be used to stop the bleeding. Also, the mouse genome has been extensively characterized, and gene-targeted (i.e., knockout) and transgenic overexpression experiments are more commonly performed using mice rather than rats.

 

Summary
  • Cardiovascular surgery focuses on the heart and blood vessels. Cardiac surgery is mainly performed to treat the complexities of ischemic heart diseases, congenital heart diseases, valvular heart diseases, endocarditis, rheumatic heart diseases, and atherosclerosis.
  • Mouse models have gained popularity because of their small size, rapid gestation age (21 days), relatively low husbandry costs, convenience in housing and handling, and fewer compound requirements for pharmacological studies.
  • With the advancement of genetic modifications to the whole organism or the myocardium and an array of biological and synthetic materials, there is an excellent potential for any combination of these to mitigate the extent of the acute ischemic injury and impede the onset of heart failure pursuant to myocardial remodeling.
  • Do not administer pre-surgical analgesia (buprenorphine) since narcotic analgesics are known to depress the respiratory center and may interfere with the survival after the open-chest surgery.
  • The significant challenges that arise during cardiac surgery are the extremely thin and delicate walls of the pulmonary trunk and the incapacity of the right ventricle to undergo stress while the pulmonary artery is being manipulated. Be careful during the operative procedures.

 

References

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